Definitive
digital imaging . . . optimal performance and efficiency
The ECLIPSE 90i is optimized for the capture of digital images,
providing aberration-free images that have excellent resolution
and uniform brightness throughout the view field.
The ECLIPSE 90i is a motorized unit that can be operated via
a PC, and interacts with the camera controls. Researchers
can perform digital imaging automatically while changing observation
technique and depth of focus.
The ECLIPSE 90i answers the needs of researchers working in
the field of life science, an area in which automation is
rapidly advancing |
|
|
|
High-precision
motorized focus |
|
|
|
Feedback from a linear encoder showing vertical
movement enables high-precision focus control in 0.05um increments,
and facilitates high-resolution deconvolution, confocal and
other techniques. |
|
|
|
Motorized
control in comfort |
|
In addition to motorized control using buttons on the microscope,
it is possible to control the ECLIPSE 90i and check the status
of the objective and other settings via the GUI of Nikon's
iControl software running on a PC monitor.
By using the optional Ergo Controller, researchers can adjust
focus and move* the stage as if seated in front of and operating
the actual microscope.
*When an optional motorized XY stage is mounted. |
|
|
|
Auto
adjustment interacting with the objective magnification |
|
The aperture and
field diaphragms, ND filter, and other units are automatically
set to the optimal position following a change of the objective.
The travel speed of the motorized stage* and focusing speeds
are also altered automatically in accordance with the objective's
magnification. Auto link focus automatically corrects deviations
in parfocal distance between each objective after the objective
is changed, eliminating the bother of focus adjustments.
*When an optional motorized XY stage is mounted. |
|
|
|
Auto
switching of observation technique |
|
Complicated procedures for changing observation
technique, such as from DIC to epi-fluorescence, are a thing
of the past. Now, changes can be made automatically by clicking
the desired technique on the GUI of a PC monitor. Setting
preferences can also be registered for each individual user,
and quickly recalled anytime. |
|
|
|
Greater
efficiency in combination with Digital Sight-series digital
cameras |
|
Interactive control of image capture
and observations |
|
It is possible to boot
the iControl software with a mouse click on the GUI of the
ACT-2U camera control software. This enables the control of
imaging in synchronization with the microscope operation.
It also facilitates imaging while changing the depth of focus
and epi-fluorescence filter, or time-lapse recording that
is carried out by changing the technique. |
|
|
|
Capture of auto-focus images |
|
In configuration with a Digital Sight-series
digital camera, the system automatically uses the image's
contrast information to enable auto-focus image capture under
the brightfield method. |
|
|
|
Auto recording of microscope status |
|
The camera automatically records the microscope
settings, including the objective magnification and fluorescence
filter in use, as a text file along with the captured image,
increasing the efficiency of managing the history log of the
images. |
|
|
|
Optimal
optics for digital imaging |
|
"Fly-eye" optics ensure uniform illumination
A revolutionary "fly-eye" lens array built into the transmitted-light
illumination optics delivers illumination with uniform brightness
throughout the visual field-perfect for digital imaging. |
|
|
|
|
Viewed
with fly-eye lens |
|
Viewed
with ordinary lens |
|
|
|
|
New VC
objectives |
|
Plan Apo VC objectives successfully correct
chromatic aberration up to the edges of the view field to
create digital images with excellent resolution and uniform
brightness- afeature perfect for tiling the captured images.
These objectives are also suitable for confocal microscopy
as they correct axial chromatic aberration even at the h-line
(405nm). |
|
|
|
New universal
epi-fluorescence illuminator |
|
Nikon's revolutionary Noise Terminator eliminates
stray light inside the filter cube to achieve a signal-to-noise
(S/N) ratio five times that of Nikon’s previous fluorescence
system. This enables weakly fluorescing specimens to be captured
with greater clarity, brightness, and dynamic range. The optional
Excitation Balancer can continuously change the spectral intensity
of each excitation wavelength of a multistained specimen.
|
|
|
|
New DIC system
The prism in the new DIC system is made of a material of a
new composition. This contributes to the creation of high-contrast
DIC images with excellent resolution and uniform coloration
regardless of which magnification is selected. |
|
|
|
Perfect
digital-imaging heads |
|
Nikon has developed two types of digital-imaging
heads integrating a Hi S/N epi-fluorescence illuminator, binocular
eyepiece tube, dual ports, and optical zoom, into a single
unit. When used in combination with a Digital Sight-series
digital camera, researchers can check status data, such as
fluorescence filter in use, zoom magnification and optical
path, on the monitor. As data can be recorded with the image
file, this feature is extremely convenient for managing the
histories of large quantities of images taken at different
settings. The motorized model DIH-E can be operated from a
PC, while the manual DIH-M comes with a motorized control
specifically for the excitation light shutter. |
|
|
|