The InCyt Im1TM Imaging System includes
everything needed for single excitation, single emission fluorophore
experiments. The system includes: an inverted epi-fluorescence/phase
contrast microscope, low-light level integrating CCD camera, 300W
Argon lamp, image processing computer and data acquisition/analysis
The InCyt Im1TM Imaging System was
designed in a biomedical research laboratory by scientists for scientists.
Menu selection appears in the sequence in which experiments are
performed. Esoteric options that add complexity and are rarely used
have been eliminated from the menus. The software follows standard
Windows graphical-user-interface-protocol. The hardware has been
tested for reliability and ruggedness, as well as, simplicity of
set up and operation. New users can learn to use the system and
be doing their first experiment in a few hours.
Data Collection, Analysis &
Data can be measured continuously
on as many as 50 preselected regions of interest. The results can
displayed in real time or images can be saved for post hoc analysis.
Saved images can be played back as an animated sequence?ideal for
analysis of subcellular responses, intercellular communication and
heterogeneity among the individual cells in a population. Ion concentration
within cells can be converted to color using standard or custom
pseudocolor tables. Image sequences can be presented as a montage
with user-defined annotation for presentation and publication. Data
generated by InCyt Im1TM are stored in a standard TIFF format and
data files are stored as ASCII text.