InCyt Im1TM Imaging System

 

 

Complete Turnkey System

The InCyt Im1TM Imaging System includes everything needed for single excitation, single emission fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, low-light level integrating CCD camera, 300W Argon lamp, image processing computer and data acquisition/analysis software.

Ease-of-use

The InCyt Im1TM Imaging System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours.

Data Collection, Analysis & Presentation

Data can be measured continuously on as many as 50 preselected regions of interest. The results can displayed in real time or images can be saved for post hoc analysis. Saved images can be played back as an animated sequence?ideal for analysis of subcellular responses, intercellular communication and heterogeneity among the individual cells in a population. Ion concentration within cells can be converted to color using standard or custom pseudocolor tables. Image sequences can be presented as a montage with user-defined annotation for presentation and publication. Data generated by InCyt Im1TM are stored in a standard TIFF format and data files are stored as ASCII text.

 

  Specifications

 Video System

 
Camera
Monochrome integrating CCD, RS170 or CCIR standard
Excitation
300W Argon Gas vis (standard)

Option: 150 watt Quartz-Halogen vis

Option: 200W Mercury lamp uv/vis

Option: 300W Xenon arc lamp uv/vis

All lamps have variable intensity

Image Resolution
640 x 480 pixels; 8-bits

 

        Data Acquisition and Analysis Workstation

CPU
800 MHz Pentium III
RAM
128 MB
Hard Drive
30 GB
CD ROM
12x
Operating System
Windows NTR
Monitor
15" SVGA

 

                    Data Capture and Display

Maximum Speed (640x480 images)
10 single-wavelength images per second
Object Definition
Specify up to 50 user-defined regions for separate analysis
Data Collection Options
(1) Graph ion concentration in each cell during experiment.
(2) Save images for later analysis and animated playback
Image Format
TIFF
Data Storage Format
Tab-delimited ASCII

 

                                 Other

Calibration From standard solutions, formula, or in situ
Ions Works with hundreds of single-excitation, single-emission fluorescent dyes

 

  Data Presentation
  • Animated playback of images
  • Montages of selected images with user-defined annotation
  • Graphs of ion kinetics in user-defined regions
  • Display images and data as raw fluorescence readings or as changes from initial state

Sample Data

  • InCyt Im1TM Data
  • ASCII Data