InCyt Im2™ Imaging System

 

Complete Turnkey System

The InCyt Im2TM Imaging System includes everything needed for calcium and other ratiometric fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, low-light level integrating CCD camera, computer controlled filter changer, Xenon UV/VIS arc lamp, image processing computer and data acquisition/analysis software.

Ease-of-use

The InCyt Im2™ Imaging System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours.

Data Collection, Analysis & Presentation

Data can be measured continuously on as many as 50 preselected regions of interest. The results can displayed in real time or images can be saved for post hoc analysis. Saved images can be played back as an animated sequence?ideal for analysis of subcellular responses, intercellular communication and heterogeneity among the individual cells in a population. Ion concentration within cells can be converted to color using standard or custom pseudocolor tables. Image sequences can be presented as a montage with user-defined annotation for presentation and publication. Data generated by InCyt Im2TM are stored in a standard TIFF format and data files are stored as ASCII text.

 

  Specifications

 Video System

 
Camera
Monochrome integrating CCD, RS170 or CCIR standard
Excitation
300 watt Xenon arc lamp for both UV and visible light excitation.
Variable intensity
Filter Changer
Computer-controlled dual filter holder with shutter
Maximum speed: 15 cycles/second
Image Resolution
640 x 480 pixels; 8-bits

 

   Data Acquisition and Analysis Workstation

CPU
2.40 Ghz Pentium 4 
RAM
256 MB
Hard Drive
40 GB
CD ROM
40x CD R/W
Operating System
Windows XP
Monitor
17" SVGA

 

              Data Capture and Display

Maximum Speed (640x480 images)
5 ratioed images per second
10 single-wavelength images per second
Object Definition
Specify up to 50 user-defined regions for separate analysis
Data Collection Options
(1) Graph ion concentration in each cell during experiment.
(2) Save images for later analysis and animated playback
Image Format
TIFF
Data Storage Format
Tab-delimited ASCII

 

                           Other

Calibration
From standard solutions or formula
Ions
Works with hundreds of fluorescent dyes for measuring: Ca2+, pH, Na+, K+, Mg+2, H2O2, cyclic ADP Ribose, etc.

 

  Data Presentation
  • Animated playback of images
  • Montages of selected images with user-defined annotation
  • Graphs of ion kinetics in user-defined regions

Sample Data

  • InCyt Im2TM Data
  • ASCII Data