Super Resolution Imaging System


Super-resolution at the speed of life!

N-SIM nearly double the resolution of conventional optical microscopes, by combining "Structured Illumination Microscopy" technology licensed from UCSF with Nikon's renowned CFI Apo TIRF 100x oil objective lens (N.A. 1.49), and it realizes high-resolution of 100nm.


TIRF-SIM imaging technique


The newly developed TIRF-SIM illumination technique enables Total Internal Reflection Fluorescence (TIRF) observation with higher resolution than conventional TIRF microscopes and gives more detailed structural information near the cell membrane.

Plasma membrane of B16 cell labeling YFP. Vesicles are observed clearly by N-SIM


3D SIM capable


The new 3D-SIM illumination technique has the capability of optical sectioning of specimens, enabling the visualization of more detailed cell structures at higher spacial resolutions up to 300nm in thickness.

Mitochondria of NIH-3T3 cell labeling MitoTrackerRed. The Crista is observed clearly by N-SIM


Time resolution of 0.6 sec/frame, the fastest in the industry


N-SIM provides the fastest imaging in the industry, with a time resolution of up to 0.6 sec/frame*, effective for live-cell imaging.

* In 2D-SIM/TIRF-SIM mode



Super high-resolution up to 20nm!

N-STORM is a new super-resolution microscope system that combines "STochastic Optical Reconstruction Microscopy" technology and Nikon's Eclipse Ti research inverted microscope. N-STORM provides dramatically enhanced resolution that is 10 times or better than that of conventional optical microscopes and will be capable of multi-spectral two-dimensional and three-dimensional nanoscopy, with lateral resolution to approximately 20nm and axial resolution to approximately 50nm, extending the role of the optical microscope to near molecular level resolution.


Three-dimensional information capability


"STORM" reconstructs nanoscale resolution fluorescence images in 3D from localization information of fluorophores detected in the z-axis with high accuracy and calculated from multiple exposures. Just as single molecules can be localized in the x-y dimension, the N-STORM 3D component can localize the same single molecule along the z-axis providing high accuracy 3D super-resolution imaging.


Unique multi-color technology


An assortment of organic dyes enables multi-color imaging. These are unique probes which are photo-activated at a shorter wavelength with respect to their peak excitation and emission. Initially these probes are in the inactivated state, but then they are excited by radiation of a specific wavelength of light, allowing them to fluorescence. This allows each fluorophore to be optically resolvable from the rest so multiple images can be compiled for the final image.

Fluorescence labeled microtuble images (Plan Apo 100x Oil)