Inverted Research Microscope
ECLIPSE Ti

New inverted microscope series at the center of bioscience's most advanced imaging techniques.

 

image Scientists have overcome many live cell imaging challenges using advanced techniques such as TIRF, confocal, FRET, photo activation and microinjection. At the center of all this is the Eclipse Ti, a powerful new system that provides instant access to all these methods plus revolutionary Nikon CFI60 optics. Available in three models, the new Ti series offers improved system speeds, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging.

 
 High-speed motorized control and acquisition
 

The operational speeds of motorized components such as the nosepiece, fluorescence filters and stage have been greatly enhanced, allowing high-speed screening image capture during multi-dimensional experiments. Faster device movement and image acquisition reduce overall light exposure and subsequent photo-toxicity, leading to more meaningful data. The newly developed digital Controller Hub significantly increases motorized accessory speed by reducing communication overhead time between components, boosting total operation speed.

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Multipoint snapshots of HeLa cells transiently expressing Venus-tubulin and mCherry-actin and stained with Hoechst33342 and DiD. (All in pseudo-color)
Photos courtesy of: Kenta Saito and Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University

 
 Stable time-lapse imaging with automatic focus correction system
 

The Ti-E comes with a unique Perfect Focus System (PFS) that automatically corrects focus drift in real time during a prolonged period of time-lapse imaging.
Focus drift resulting from a temperature drop when reagents are added is instantaneously corrected and the rapid change of cells can be captured.
The incorporation of the PFS in the nosepiece unit saves space and allows two optical component levels to be attached simultaneously utilizing stratum structure.

Concept of the Perfect Focus System image

The diagram shows the case when an immersion type objective is used. A dry type objective is also available.

 

 

 

Correction to focus drift when reagents are added

With PFS
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Without PFS
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 High-quality phase contrast images using high NA lenses
 

image The revolutionary external phase contrast unit incorporates a phase ring and allows the use of high NA objective lenses without a phase ring for phase contrast observation. Because there is no light loss due to a phase ring, bright "full intensity" fluorescence images as well as high-resolution phase contrast images can be captured using the same objective lens.

 
 Advanced integration with peripherals via intelligent software
 

Nikon's comprehensive imaging software NIS-Elements provides an integrated control of the microscope, cameras, components and peripherals. The intuitive GUI and efficient workflow make the programming of automated imaging sequences up to 6D (X, Y, Z, time, wavelength, multipoint) easy to perform.

 

6D time-lapse imaging system image

 
 Motorized laser TIRF illumination unit
 

image The motorized laser TIRF illuminator is newly developed for Ti-E and Ti-U. The laser incident angle can be easily stored and reproduced with a single touch of the control pad button.
This enables alternate time-lapse recording between multi-wavelength TIRF and epi-fluorescence images.

Time-lapse imaging by switching TIRF and epi-fluorescence observation
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NG108 cell: Growth cone stained with EGFP-fascin
Photos courtesy of: Satoe Ebihara, Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST)

 
 Photo activation illumination unit
 

image The Ti-E and Ti-U feature a specialized photo activation illuminator that allows fluorescent time-lapse observation of dynamic events following photo activation or photo conversion.

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Photo activation of PA-GFP in a living mammalian cell by 405nm laser irradiation
Photos courtesy of: Tomoki Matsuda and Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University

 
 Multiport design with a maximum of five imaging ports
 
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Use of an optional back port enables multiple wavelength FRET imaging with multiple cameras. Moreover, by adding an eyepiece tube base unit with a side port, a maximum of five imaging ports* including left, right and bottom ports are available. (*With Ti-E/B model with bottom port)

 

 

 

 
 Three models for a wide range of applications
 
imageTi-E

The flagship model that is fully motorized for automated multimode image techniques and acquisition
(A model with a bottom port is also available.)

 

image Ti-U

The universal model with the potential for diverse laser illuminators and motorized components
(A model with a bottom port is also available.)

 

image Ti-S

The basic model that can be dedicated to specific tasks

 

 

data sheet (*.pdf)