Finally a culture dish system specifically
designed for live-cell microscopy! Now you can have accurate temperature
control and high numeric aperture compatibility in a convenient
disposable culture dish system that even works in confocal applications.
- Easily adapted to a variety of specimen
types from monolayered adherent cells to brain slice and tissue
- Low mass to thermo-regulate as opposed
to conventional stage heaters
- Plate, incubate and observe without the
need to transfer your cells
- Fast thermal recovery after perfusion
- Compatible with inverted and upright microscope
- Coverglass bottom for optimum optical
- No need for warm air blowers or stage
- Direct first-surface heating to your cells
- Can also be cooled below ambient
- Perfusion available
- No preheating
Bioptechs is now introducing the next generation
Open Culture Dish Micro-Environmental Control System: the Delta
T4. In addition to the improvements to the popular Delta TC3 control
algorithm, Bioptechs has incorporated years of customer request
into the Delta T4. Click here for a description of features.
The Bioptechs, Inc. Delta T Culture Dish System is designed to simulate
host conditions on the stage of your microscope and provide an optimal
optical environment for microscopy. This two-step system allows
you to plate your cells and observe them without having to transfer
them to another structure. The system components are, the Controller,
Stage Adapter, and Dishes. Accessories for Tissue Slice, Brain Slice
and other specimens are available.
An intelligent feedback loop passes an electrical
current through a thin film coating on the underside of the glass
substrate on which the cells are grown. Heat is applied directly
to the cells without the inefficiencies associated with peripheral
heating by traditional culture dish warmers. Bioptechs exclusively
offers opaque culture dishes which eliminates the unwanted ambient
light background for fluorescence imaging.
The controller features a real-time temperature
display and fast learning curve to compensate for cooling due to
surface evaporation while responding to temperature changes due
to perfusion. There is also an alarmed protection circuit to safeguard
the cells and an internal reference for the user adjustable calibration.
The standard controller has a temperature range of ambient to 50
degrees C, extended ranges are available upon request.
Delta T dishes have 35mm O.D. and a 23mm
central aperture, the peripheral region of the dish is tapered to
reduce the dead-volume and the height of the dish is 6mm to allow
better access for micro-injection and micromanipulation. The dishes
are a hybrid of polystyrene plastic and Dasog 243 glass. The outer
structure of the dish is available in opaque black or clear and
come with a clear 0.5mm or no.1.5 glass coverslip bottom bonded
to it. The dishes are also available in a plain glass unheated version.