InCyt Cv2TM Spectrometer


Complete Turnkey System

The InCyt Cv2TM spectrometer includes everything needed for calcium and other ratiometric fluorophore experiments. The system includes: L-format sample compartment with micro-stirrer, filter holders, water jacketed cuvette holder and mechanical shutter, photon counting photometer assembly, computer controlled filter changer, Xenon UV/VIS arc lamp, data acquisition & analysis computer workstation and data acquisition/analysis software.


The InCyt Cv2TM spectrometer was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours. The sample compartment is designed to offer maximum accessibility for customizing perfusion and sample handling.

Data Collection, Analysis & Presentation

Data can be measured continuously in user definable regions of interest. The results are displayed in real time and can be saved for post hoc analysis. Ion concentration within cells or ratio values can be displayed. Data files generated by InCyt Cv2TM are stored as ASCII text.


 Video System

Hamamatsu HC-135 photon counter
Discriminator & microprocessor
built into PMT
Automatic PMT Shutter
optional (mechanical shutter standard)
Excitation Source
300 W XE arc lamp UV/VIS illuminator
Filter Changer
computer controlled dual filter holder with shutter, maximum speed 25 cycles per second
15" SVGA


                   Data Processing Unit

800 MHz Pentium III
64 MB
Operating System
Windows NT
Hard Drive
30 GB
Floppy Drive
3.5" high-density


                   Data Capture and Display

Maximum Speed
25 ratioed measurements per second or 200 single wavelength per second
Time Lapse
up to 1 measurement/10 minutes
Field of View
rectangular, user definable
Graphic Display
realtime during experiment
Data Storage Format


  • Calibration from standards, formula, or in situ
  • Works with any of the hundreds of single excitation or dual excitation fluorophores