The InCyt Cv2TM spectrometer includes everything
needed for calcium and other ratiometric fluorophore experiments.
The system includes: L-format sample compartment with micro-stirrer,
filter holders, water jacketed cuvette holder and mechanical shutter,
photon counting photometer assembly, computer controlled filter
changer, Xenon UV/VIS arc lamp, data acquisition & analysis
computer workstation and data acquisition/analysis software.
The InCyt Cv2TM spectrometer was designed in a biomedical
research laboratory by scientists for scientists. Menu selection
appears in the sequence in which experiments are performed. Esoteric
options that add complexity and are rarely used have been eliminated
from the menus. The software follows standard Windows graphical-user-interface-protocol.
The hardware has been tested for reliability and ruggedness, as
well as, simplicity of set up and operation. New users can learn
to use the system and be doing their first experiment in a few hours.
The sample compartment is designed to offer maximum accessibility
for customizing perfusion and sample handling.
Data Collection, Analysis &
Data can be measured continuously in user definable
regions of interest. The results are displayed in real time and
can be saved for post hoc analysis. Ion concentration within cells
or ratio values can be displayed. Data files generated by InCyt
Cv2TM are stored as ASCII text.