InCyt I/P-1™ Imaging & Photometry System

 

Complete Turnkey System

The InCyt I/P-1TM Imaging System includes everything needed for single excitation, single emission fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, microphotometer assembly, low-light level integrating CCD camera, 150W quartz-halogen lamp, image processing computer and data acquisition/analysis software.

Ease-of-use

The InCyt I/P-1TM Imaging System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours.

Data Collection, Analysis & Presentation

Imaging data can be measured continuously on as many as 50 preselected regions of interest. The results can displayed in real time or images can be saved for post hoc analysis. Saved images can be played back as an animated sequence?ideal for analysis of subcellular responses, intercellular communication and heterogeneity among the individual cells in a population. Ion concentration within cells can be converted to color using standard or custom pseudocolor tables. Image sequences can be presented as a montage with user-defined annotation for presentation and publication. Data generated by InCyt I/P-1TM are stored in a standard TIFF format and data files are stored as ASCII text.


  Specifications

 Video System

 
Camera
Monochrome 12 bit CCD
Excitation
150 watt Quartz-Halogen (standard) for visible light excitation

Option: 300 watt Argon Gas (visible light)

Option: 150 watt Mercury lamp (UV/visible)

Option: 300 watt Xenon arc lamp (UV/visible)

All lamps have variable intensity

Image Resolution
640 x 480 pixels; 12-bits

                 Photometer System

PMT
Hamamatsu HC135-11 photon counter
Linearity (precision)
+/-1% from 0 to 20,000,000 counts/second
Maximum Dark Count (noise)
150 counts/second
Equivalent Noise Input (noise)
3 x 10-17 watts per second (measured at 400nm)
Spectral Sensitivity
300-650nm
Discriminator & Microprocessor
Built into PMT
Overload Protection
Computer-controlled shutter (optional)
Excitation
150 watt Quartz-Halogen (standard) for visible light excitation

Option: 300 watt Argon Gas (visible light)

Option: 150 watt Mercury lamp (UV/visible)

Option: 300 watt Xenon arc lamp (UV/visible)

All lamps have variable intensity

Image/Data Acquisition and Analysis Workstation

CPU
2.40 Ghz Pentium 4
RAM
256 MB
Hard Drive
40 GB
CD ROM
40x CD R/W
Operating System
Windows XP
Monitor
17" SVGA

 

   Image Data Capture and Display

Maximum Speed (640x480 images)
10 single-wavelength images per second
Object Definition
Specify up to 50 user-defined regions for separate analysis
Data Collection Options
(1) Graph ion concentration in each cell during experiment.
(2) Save images for later analysis and animated playback
Image Format
TIFF
Data Storage Format
Tab-delimited ASCII

 

        PMT Data Capture and Display

Maximum Speed
100 single-wavelength measurements per second
Time Lapse
Up to 1 measurement every 10 minutes
Field of View
Rectangular area, user-definable
Graphic Display
Real-time during experiment
Data Storage Format
Tab-delimited ASCII

 

                         Other

Calibration
From standard solutions or formula
Ions
Works with hundreds of single-excitation, single-emission fluorescent dyes

 

 

Imaging Data Presentation
  • Animated playback of images
  • Montages of selected images with user-defined annotation
  • Graphs of ion kinetics in user-defined regions
  • Display images and data as raw fluorescence readings or as changes from initial state

Sample Data

  • InCyt I/P-1TM Imaging Data
  • ASCII Data
  • InCyt I/P-1TM Photometry Data