The InCyt I/P-2TM Imaging System includes everything needed for
calcium and other ratiometric fluorophore experiments. The system
includes: an inverted epi-fluorescence/phase contrast microscope,
microphotometer assembly, low-light level integrating CCD camera,
computer controlled filter changer, Xenon UV/VIS arc lamp, image
processing computer and data acquisition/analysis software.
InCyt I/P-2TM Imaging System was designed in a biomedical research
laboratory by scientists for scientists. Menu selection appears
in the sequence in which experiments are performed. Esoteric options
that add complexity and are rarely used have been eliminated from
the menus. The software follows standard Windows graphical-user-interface-protocol.
The hardware has been tested for reliability and ruggedness, as
well as, simplicity of set up and operation. New users can learn
to use the system and be doing their first experiment in a few hours.
Collection, Analysis & Presentation
data can be measured continuously on as many as 50 preselected regions
of interest. The results can displayed in real time or images can
be saved for post hoc analysis. Saved images can be played back
as an animated sequence?ideal for analysis of subcellular responses,
intercellular communication and heterogeneity among the individual
cells in a population. Ion concentration within cells can be converted
to color using standard or custom pseudocolor tables. Image sequences
can be presented as a montage with user-defined annotation for presentation
and publication. Data generated by InCyt I/P-2TM are stored in a
standard TIFF format and data files are stored as ASCII text.