InCyt Pm2TM Photometry System


  Complete Turnkey System

The InCyt Pm2TM Photometry System includes everything needed for calcium and other ratiometric fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, photon counting photometer assembly, computer controlled filter changer, Xenon UV/VIS arc lamp, data acquisition & analysis computer workstation and data acquisition/analysis software.


The InCyt Pm2TM Photometry System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours.

Data Collection, Analysis & Presentation

Data can be measured continuously in user definable regions of interest. The results are displayed in real time and can be saved for post hoc analysis. Ion concentration within cells or ratio values can be displayed. Data files generated by InCyt Pm2TM are stored as ASCII text.



Photometer System

Hamamatsu HC135-11 photon counter
Linearity (precision)
+/-1% from 0 to 20,000,000 counts/second
Maximum Dark Count (noise)
150 counts/second
Equivalent Noise Input (noise)
3 x 10-17 watts per second (measured at 400nm)
Spectral Sensitivity
Discriminator & Microprocessor
Built into PMT
Overload Protection
Computer-controlled shutter (optional)
300 watt Xenon arc lamp for both UV and visible light excitation.
Variable intensity
Filter Changer
Computer-controlled dual filter holder with shutter
Maximum speed: 15 cycles/second


       Data Acquisition and Analysis Workstation

800 MHz Pentium III
64 MB
Hard Drive
10 GB
Operating System
Windows NT
15" SVGA


                 Data Capture and Display

Maximum Speed
14 ratioed measurements per second
100 single-wavelength measurements per second
Time Lapse
Up to 1 measurement every 10 minutes
Field of View
Rectangular area, user-definable
Graphic Display
Real-time during experiment
Data Storage Format
Tab-delimited ASCII



From standard solutions, formula, or in situ
Works with hundreds of fluorescent dyes for measuring: Ca2+, pH, Na+, K+, Mg+2, H2O2, cyclic ADP Ribose, etc.