InCyt Pm2TM Photometry System

 

  Complete Turnkey System

The InCyt Pm2TM Photometry System includes everything needed for calcium and other ratiometric fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, photon counting photometer assembly, computer controlled filter changer, Xenon UV/VIS arc lamp, data acquisition & analysis computer workstation and data acquisition/analysis software.

Ease-of-use

The InCyt Pm2TM Photometry System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours.

Data Collection, Analysis & Presentation

Data can be measured continuously in user definable regions of interest. The results are displayed in real time and can be saved for post hoc analysis. Ion concentration within cells or ratio values can be displayed. Data files generated by InCyt Pm2TM are stored as ASCII text.

 

  Specifications

Photometer System

 
PMT
Hamamatsu HC135-11 photon counter
Linearity (precision)
+/-1% from 0 to 20,000,000 counts/second
Maximum Dark Count (noise)
150 counts/second
Equivalent Noise Input (noise)
3 x 10-17 watts per second (measured at 400nm)
Spectral Sensitivity
300-650nm
Discriminator & Microprocessor
Built into PMT
Overload Protection
Computer-controlled shutter (optional)
Excitation
300 watt Xenon arc lamp for both UV and visible light excitation.
Variable intensity
Filter Changer
Computer-controlled dual filter holder with shutter
Maximum speed: 15 cycles/second

 

       Data Acquisition and Analysis Workstation

CPU
800 MHz Pentium III
RAM
64 MB
Hard Drive
10 GB
CD ROM
12x
Operating System
Windows NT
Monitor
15" SVGA

 

                 Data Capture and Display

Maximum Speed
14 ratioed measurements per second
100 single-wavelength measurements per second
Time Lapse
Up to 1 measurement every 10 minutes
Field of View
Rectangular area, user-definable
Graphic Display
Real-time during experiment
Data Storage Format
Tab-delimited ASCII

 

                               Other

Calibration
From standard solutions, formula, or in situ
Ions
Works with hundreds of fluorescent dyes for measuring: Ca2+, pH, Na+, K+, Mg+2, H2O2, cyclic ADP Ribose, etc.